Quinoline Biodegradation by Bacillus Licheniformis Strain CRC-75

Quinoline Biodegradation by Bacillus Licheniformis Strain CRC-75

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A bacterial culture was isolated from oil-contaminated soil based on its ability to metabolize the quinoline as the sole source of nitrogen.In this research Bacillus licheniformis strain was identified based on the result of Omega 7 16S rRNA analysis.Optimized conditions were obtained with full factorial experimental design method using design expert software at temperature: 32oC, agitation speed: 200rpm, quinoline concentration: 400 ppm.The efficiency of Bacillus licheniformis for biodegradation of quinoline at the optimum conditions was determined to be 35%.

Two-phase cultivation media were used with 5, 10, 15 and 20% heavy crude oil concentration in aqueous media.Resting cells Pin Connector Crimp Terminals of Bacillus licheniformis was shown to be capable of removing about 25% of total nitrogen in 5% heavy crude oil.GC analysis showed a decreasing trend in the activity of this strain against crude oil concentration.